Active Recombinant Human CLK4 Protein, GST-tagged

• Cat.No.: CLK4-1500H
• Product Overview: Human CLK4 (NP_065717.1, 1 a.a. - 481 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined. [provided by RefSeq, Jul 2008]
• Form: Liquid
• Bio-activity: The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: SRPKtide. ATP: 100 uM.
• Molecular Mass: 85 kDa
• Purity: 87 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CLK4 CDC-like kinase 4 [ Homo sapiens ]
• Official Symbol: CLK4
• Synonyms: CLK4; CDC-like kinase 4; dual specificity protein kinase CLK4; protein serine threonine kinase Clk4; DKFZp686A20267
• Gene ID: 57396
• mRNA Refseq: NM_020666
• Protein Refseq: NP_065717
• MIM: 607969
• UniProt ID: Q9HAZ1