Recombinant Human CLC Protein (Ser2-Arg142), N-His tagged, Animal-free, Carrier-free

• Cat.No.: CLC-13H
• Product Overview: Recombinant Human CLC Protein (Ser2-Arg142) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Galectin-10 (Gal-10) is a lectin family member and is one of the prototype galectins. It contains one carbohydrate recognition domain (CRD), responsible for β-galactoside binding, and is biologically active as homodimers. It has been reported that galectin-10 is presented in eosinophils but also detected in basophils and macrophages. Accumulated evidence indicates that galectin-10 spontaneously forms Charcot-Leyden crystals (CLCs), participating in allergic responses and related inflammatory reactions. Several binding partners for galectin-10 have been identified, including eosinophil granule cationic ribonucleases, eosinophil-derived neurotoxin (EDN, RNS2), and eosinophil cationic protein (ECP, RNS3), demonstrating the interaction is essential for eosinophil differentiation and granulogenesis.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 36.7 kDa.
  The protein migrates as 37 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Ser2-Arg142
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: CLC Charcot-Leyden crystal protein [ Homo sapiens (human) ]
• Official Symbol: CLC
• Synonyms: CLC; Charcot-Leyden crystal protein; eosinophil lysophospholipase; galectin 10; LGALS10; LPPL_HUMAN; lysolecithin acylhydrolase; MGC149659; galectin-10; GAL10; Gal-10; LGALS10A
• Gene ID: 1178
• mRNA Refseq: NM_001828
• Protein Refseq: NP_001819
• MIM: 153310
• UniProt ID: Q05315