Recombinant Human CHRNB4 Protein, His-tagged

• Cat.No.: CHRNB4-782H
• Product Overview: Recombinant Human CHRNB4 Protein (Arg22-Thr236) with His tag was expressed in E. coli.

Specification

• Description: This gene is found within a conserved gene cluster and encodes one of the beta subunits of the nicotinic acetylcholine receptor (nAChRs) superfamily which form ligand-gated ion channels with a central pore that forms a cation channel. Neuronal nAChRs are pentameric structures that can be either homomeric or heteromeric, with heteromeric structures containing both alpha and beta subunits. Each subunit contains an extracellular amino terminus and four transmembrane domains. Nicotine is one of the agonists that binds to the receptor. Variants in this gene have been associated with nicotine dependence and lung cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.8 kDa; Accurate Molecular Mass: 32 kDa
• Protein length: Arg22-Thr236
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Gene Information

• Gene Name: CHRNB4 cholinergic receptor, nicotinic, beta 4 (neuronal) [ Homo sapiens (human) ]
• Official Symbol: CHRNB4
• Synonyms: CHRNB4; cholinergic receptor, nicotinic, beta 4 (neuronal); cholinergic receptor, nicotinic, beta polypeptide 4; neuronal acetylcholine receptor subunit beta-4; acetylcholine receptor; nicotinic; beta 4 (neuronal); neuronal nicotinic receptor beta 4 subunit; acetylcholine receptor, nicotinic, beta 4 (neuronal)
• Gene ID: 1143
• mRNA Refseq: NM_000750
• Protein Refseq: NP_000741
• MIM: 118509
• UniProt ID: P30926