Recombinant Human CHN1 protein, His&GST-tagged

• Cat.No.: CHN1-0255H
• Product Overview: Recombinant Human CHN1 protein(NP_001813.1)(Ala2-Phe459), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 2-459 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant human CHN1/GST chimera consists of 695 amino acids and has a calculated molecular mass of 80.9 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Ala2-Phe459
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CHN1 chimerin (chimaerin) 1 [ Homo sapiens ]
• Official Symbol: CHN1
• Synonyms: CHN1; chimerin (chimaerin) 1; CHN, Duane retraction syndrome 2 , DURS2; N-chimaerin; ARHGAP2; Chimerin 1 (GTPase activating protein; rho; 2); n chimerin; RhoGAP2; n-chimerin; A-chimaerin; a2-chimaerin; alpha-chimerin; Rho GTPase-activating protein 2; NC; CHN; DURS2; RHOGAP2
• Gene ID: 1123
• mRNA Refseq: NM_001025201
• Protein Refseq: NP_001020372
• MIM: 118423
• UniProt ID: P15882