Recombinant Human Chloride Intracellular Channel 1, His-tagged

• Cat.No.: CLIC1-2498H
• Product Overview: Recombinant human CLIC1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 29.0 kDa (261aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CLIC1 (Chloride intracellular channel 1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. This protein is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as an anion-selective channel. It forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP GGQLPFLLYG TEVHTDTNKI EEFLEAVLCP PRYPKLAALN PESNTAGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GVSQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVAKAL K.
• Storage: can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CLIC1 chloride intracellular channel 1 [ Homo sapiens ]
• Synonyms: chloride intracellular channel 1; G6; NCC27; CLIC1; chloride intracellular channel protein 1; hRNCC; p64CLCP; RNCC protein; OTTHUMP00000029133; OTTHUMP00000029137; OTTHUMP00000174486; chloride channel ABP; nuclear chloride ion channel 27; nuclear chloride ion channel protein; regulatory nuclear chloride ion channel protein;Chloride channel ABP; Nuclear chloride ion channel 27; Regulatory nuclear chloride ion channel protein
• Gene ID: 1192
• mRNA Refseq: NM_001288
• Protein Refseq: NP_001279
• MIM: 602872
• UniProt ID: O00299
• Chromosome Location: 6p22.1-p21.2
• Function: chloride channel activity; protein binding; voltage-gated chloride channel activity; voltage-gated ion channel activity