Recombinant Human CHI3L2 protein(Met1-Leu390), His-tagged

• Cat.No.: CHI3L2-1177H
• Product Overview: Recombinant Human CHI3L2 (NP_003991.2) (Met 1-Leu 390) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-390 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CHI3L2 consists of 374 amino acids and has a predicted molecular mass of 42.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCHI3L2 is approximately 43 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CHI3L2 chitinase 3-like 2 [ Homo sapiens ]
• Official Symbol: CHI3L2
• Synonyms: CHI3L2; chitinase 3-like 2; chitinase-3-like protein 2; YKL 39; YKL39; chondrocyte protein 39; YKL-39
• Gene ID: 1117
• mRNA Refseq: NM_001025197
• Protein Refseq: NP_001020368
• MIM: 601526
• UniProt ID: Q15782

Case Study

Case 1: Ranok A, et al. J Biol Chem. 2015

Scientists have delved into how human YKL-39 hooks onto chitooligosaccharides through hydrogen bonds and hydrophobic forces. It has a unique barrel shape with a short groove for sugars, featuring five key spots, with Trp-360 playing a pivotal role. It binds stronger as the sugar chain lengthens, driven mainly by enthalpy.

Fig1. Refined structure of YKL-39 in complex with GlcNAc6.

Fig2. YKL-39 ligand binding studied by intrinsic fluorescence spectroscopy.

Case 2: Qian W, et al. J Gene Med. 2023

Researchers looked into CHI3L2's role in gliomas using RNA-seq, proteomics, and other methods. They found that CHI3L2 is more prominent in gliomas than in normal tissues, which ties to poorer survival rates. Tests like qRT-PCR and IHC backed up these results. Additionally, CHI3L2 seems to influence tumor immunity, affecting how immune cells operate within gliomas. It’s involved in several pathways, making it a promising target for new glioma treatments.

Fig1. immunohistochemistry (IHC) results of CHI3L2 protein expressions in different group.

Fig2. The umap and violin plots of CHI3L2 in glioma in GSE138794 dataset.

Application

The Recombinant CHI3L2 protein is turning heads in the science community, especially for cancer research. It's a big deal in gliomas since its levels are tied to how bad the cancer can get. By using this protein in the lab, scientists are figuring out how it can act as a signpost, helping diagnose and maybe even predict the aggressiveness of certain tumors. CHI3L2 is making waves in the immunology field. Scientists are intrigued by how it affects immune cells, offering a path to potentially boost the immune system's ability to target cancer cells more precisely. On another front, CHI3L2 is gaining attention in regenerative medicine due to its potential in aiding cell growth and repair. This could be significant for wound healing and fighting degenerative diseases, as it helps foster healthy tissue development. All in all, recombinant CHI3L2 protein is shaping up to be a promising player in upcoming medical discoveries.

Fig1. Schematic illustration of YKL-39 activity in cancer. (Julia Kzhyshkowska, 2020)