Recombinant Human CHEK1, His-tagged, Active

• Cat.No.: CHEK1-281H
• Product Overview: Recombinant human full-length CHK1 was expressed by baculovirus inSf9insect cells using an N-terminal His tag. MW=59 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CHK1 is a 56 kDa serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CHEK1 CHK1 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Synonyms: CHEK1; CHK1 checkpoint homolog (S. pombe); CHK1; CHK1 (checkpoint, S.pombe) homolog; Serine/threonine-protein kinase Chk1;EC2.7.11.1
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• UniProt ID: O14757
• Chromosome Location: 11q24.2
• MIM: 603078
• Pathway: Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity