Recombinant Human Charged Multivesicular Body Protein 6, His-tagged

• Cat.No.: CHMP6-5589H
• Product Overview: Recombinant humanCHMP6 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Cat. No.: CHMP6-5589H
• Description: CHMP6,as known as charged multivesicular body protein 6, belongs to the SNF7family. This Protein is core component of the endosomal sorting required fortransport complex III (ESCRT-III) which is involved in multivesicular bodies(MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBscontain intraluminal vesicles (ILVs) that are generated by invagination andscission from the limiting membrane of the endosome and mostly are deliveredto lysosomes enabling degradation of membrane proteins, such as stimulatedgrowth factor receptors, lysosomal enzymes and lipids.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl.
• Molecular Weight: 26.1kDa(225aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appearhigher).
• Purity: > 95%by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMGNLFG RKKQSRVTEQ DKAILQLKQQ RDKLRQYQKR IAQQLERERALARQLLRDGR KERAKLLLKK KRYQEQLLDR TENQISSLEA MVQSIEFTQI EMKVMEGLQF GNECLNKMHQVMSIEEVERI LDETQEAVEY QRQIDELLAG SFTQEDEDAI LEELSAITQE QIELPEVPSE PLPEKIPENVPVKARPRQAE LVAAS
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CHMP6 charged multivesicularbody protein 6 [ Homo sapiens ]
• Official Symbol: CHMP6
• Synonyms: CHMP6; charged multivesicular body protein6; VPS20; hVps20; Vps20; FLJ11749; chromatin modifying protein 6;chromatin-modifying protein 6; vacuolar protein sorting-associated protein 20
• Gene ID: 79643
• mRNA Refseq: NM_024591
• Protein Refseq: NP_078867
• MIM: 610901
• UniProt ID: Q96FZ7
• Chromosome Location: 17q25.3
• Pathway: ESCRT-III complex; Endocytosis; EndosomalSorting Complex Required For Transport (ESCRT); Membrane Trafficking
• Function: protein N-terminus binding