Recombinant Human CFL1 Protein, His-tagged

• Cat.No.: CFL1-768H
• Product Overview: Recombinant Human CFL1 Protein (Ser3-Leu161) with His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser3-Leu161
• Description: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.6 kDa; Accurate Molecular Mass: 22 kDa
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CFL1 cofilin 1 (non-muscle) [ Homo sapiens (human) ]
• Official Symbol: CFL1
• Synonyms: CFL1; cofilin 1 (non-muscle); CFL; cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform
• Gene ID: 1072
• mRNA Refseq: NM_005507
• Protein Refseq: NP_005498
• MIM: 601442
• UniProt ID: P23528