Recombinant Human CFD protein, His-tagged

• Cat.No.: CFD-187H
• Product Overview: Recombinant Human CFD protein(P00746-1)(Ile26-Ala253), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ile26-Ala253
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Complement Factor D, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 26-27 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CFD complement factor D (adipsin) [ Homo sapiens ]
• Official Symbol: CFD
• Synonyms: CFD; complement factor D (adipsin); D component of complement (adipsin) , DF, PFD, properdin factor D; complement factor D; ADN; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin); DF; PFD; ADIPSIN
• Gene ID: 1675
• mRNA Refseq: NM_001928
• Protein Refseq: NP_001919
• MIM: 134350
• UniProt ID: P00746