Recombinant Human Cell Division Cycle Associated 8, His-tagged

• Cat.No.: CDCA8-3526H
• Product Overview: Recombinant human CDCA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (19-280aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CDCA8, also known as Borealin, is a component of the chromosomal passenger complex (CPC). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly.In the complex, CDCA8 protein may be required to direct the CPC to centromeric DNA. Deletion of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,50% glycerol, 1mM EDTA, 0.2M NaCl
• Molecular Weight: 31.6 kDa (283aa)confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAATADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRLEVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSNRLAQICSSIR THK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CDCA8 cell division cycle associated 8 [ Homo sapiens ]
• Synonyms: CDCA8; BOR; DasraB; MESRGP; BOREALIN; FLJ10468; FLJ12042; borealin; Dasra B; dasra-B; hDasra-B; OTTHUMP00000004514; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein
• Gene ID: 55143
• mRNA Refseq: NM_018101
• Protein Refseq: NP_060571
• MIM: 609977
• UniProt ID: Q53HL2
• Chromosome Location: 1p34.3
• Function: protein binding