Recombinant Human CEACAM1 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CEACAM1-662HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CEACAM1 (Gln35-Gly428) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln35-Gly428
• Description: Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1/CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1/CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1/BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 44.2 kDa. The protein migrates as 57-100 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• N-terminal Sequence Analysis: Gln 35
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CEACAM1
• Official Symbol: CEACAM1
• Synonyms: CEACAM1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); BGP; carcinoembryonic antigen-related cell adhesion molecule 1; BGP1; CD66a; antigen CD66; CD66a antigen; BGPI
• Gene ID: 634
• mRNA Refseq: NM_001024912
• Protein Refseq: NP_001020083
• MIM: 109770
• UniProt ID: P13688