Recombinant Human CDK5/SNAP25, GST-tagged, Active

• Cat.No.: CDK5/SNAP25-276H
• Product Overview: Recombinant human full-length CDK5 and p25 were co-expressed by baculovirus inSf9insect cells using an N-terminal GST tag on both proteins. MW=59 kDa and p25 ~49 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5. CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CDK5 cyclin-dependent kinase 5 [ Homo sapiens ],SNAP25 synaptosomal-associated protein, 25kDa [ Homo sapiens ]
• Synonyms: CDK5; cyclin-dependent kinase 5; PSSALRE;Cell division protein kinase 5;EC2.7.11.22; Cyclin-dependent kinase 5; Tau protein kinase II catalytic subunit; TPKII catalytic subunit; Serine/threonine-protein kinase; SNAP25; FLJ23079; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP; Super-Protein, bA416N4.2; dJ1068F16.2; synapse protein 25,isoform A; CG40452-PA; Snap25-PA; synapse protein 25, isoform B; CG40452-PB; Snap25-PB; OTTHUMP00000030267; OTTHUMP00000030268; Super protein; Synaptosomal-associated 25 kDa protein; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein 25; synaptosomal associated protein, 25kDa; Synaptosomal-associated protein, 25kDa; SNAP-25 (synaptosome-associated protein of 25,000 daltons).EF-hand calcium binding protein; FLJ23079
• Gene ID: 1020,6616
• mRNA Refseq: NM_001164410,NM_003081
• Protein Refseq: NP_001157882,NP_003072
• UniProt ID: Q00535,P60880
• Chromosome Location: 7q36, 20p12-p11.2
• MIM: 123831,600322
• Pathway: Alzheimer"s disease; Axon guidance; Opioid Signalling; SNARE interactions in vesicular transport; Botulinum neurotoxicity; Diabetes pathways; Integration of energy metabolism; Synaptic Transmission
• Function: ATP binding; ErbB-2 class receptor binding; ErbB-3 class receptor binding; acetylcholine receptor activator activity; cyclin-dependent protein kinase activity; nucleotide binding; p53 binding; transferase activity; tau-protein kinase activity;SNAP receptor activity; SNAP receptor activity; soluble NSF attachment protein activity

Q&As

Is CDK5 derived from human fetal cell lines?

The CDK5 is expressed from HEK293 host, which is a mammalian cells system.