Recombinant Human CDK4/CCND3, GST-tagged, Active

• Cat.No.: CDK4/CCND3-275H
• Product Overview: Recombinant human full-length CDK4 and CyclinD3 were co-expressed by baculovirus inSf9cells using an N-terminal GST tag on both proteins. MW=58 kDa and CyclinD3 ~58 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ],CCND3 cyclin D3 [ Homo sapiens ]
• Synonyms: CDK4; cyclin-dependent kinase 4; CMM3; PSK-J3; MGC14458; cell division kinase 4; melanoma cutaneous malignant, 3; EC 2.7.11.22; CCND3;cyclin D3; G1/S-specific cyclin-D3
• Gene ID: 1019,896
• mRNA Refseq: NM_000075,NM_001136017
• Protein Refseq: NP_000066,NP_001129489
• UniProt ID: P11802, P30281
• Chromosome Location: 12q14, 6p21
• MIM: 123829,123834
• Pathway: Bladder cancer; Cell cycle; Chronic myeloid leukemia; Glioma; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Small cell lung cancer; T cell receptor signaling pathway; Tight junction; p53 signaling pathway; Cell Cycle, Mitotic; Jak-STAT signaling pathway;Wnt signaling pathway
• Function: cyclin-dependent protein kinase activity; ATP binding; nucleotide binding; transferase activity;protein kinase binding; protein binding