Recombinant Human CDK4/CCND1, His-tagged

• Cat.No.: CDK4/CCND1-633H
• Product Overview: Recombinant human CDK4 and Cyclin D1 (amino-acids 16 – 271) Complex of C-terminal His-tagged was Co-expressed in a Baculovirus infectedSf9 cellexpression system. CDK4, MW=34 kDa, Cyclin D1, MW=29 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 16-271 a.a.
• Description: CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80°C.
• Pathways: Bladder cancer; Cell cycle; Chronic myeloid leukemia; Glioma; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Small cell lung cancer; T cell receptor signaling pathway; Tight junction; p53 signaling pathway; Cell Cycle, Mitotic; Acute myeloid leukemia; Bladder cancer; Colorectal cancer; Endometrial cancer

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ],CCND1 cyclin D1 [ Homo sapiens ]
• Synonyms: CDK4; cyclin-dependent kinase 4; CMM3; PSK-J3; MGC14458; cell division kinase 4; melanoma cutaneous malignant, 3; EC 2.7.11.22; CCND1; cyclin D1; BCL1; PRAD1; U21B31; D11S287E; cyclin D1 (PRAD1: parathyroid adenomatosis 1); parathyroid adenomatosis 1; B-cell CLL/lymphoma 1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; BCL-1 oncogene
• Gene ID: 1019,595
• mRNA Refseq: NM_000075,NM_053056
• Protein Refseq: NP_000066,NP_444284
• MIM: 123829,168461
• UniProt ID: P11802, P24385
• Chromosome Location: 12q14, 11q13
• Function: cyclin-dependent protein kinase activity; ATP binding; nucleotide binding; transferase activity; protein complex binding; protein kinase binding