Recombinant Human CDK16 protein(Met1-Phe496), GST-tagged

• Cat.No.: CDK16-952H
• Product Overview: Recombinant Human PCTK1 (Q00536-1) (Met 1-Phe 496) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: GST
• Protein length: Met1-Phe496
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 2mM GSH, 0.5mM PMSF.
• Molecular Mass: The recombinant human PCTK1/GST chimera consists of 720 amino acids and has a predicted molecular mass of 82 kDa. It migrates as an approximately 73 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 82 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: Please refer to the datasheet for detailed information.

Gene Information

• Gene Name: CDK16 cyclin-dependent kinase 16 [ Homo sapiens ]
• Official Symbol: CDK16
• Synonyms: CDK16; cyclin-dependent kinase 16; PCTAIRE protein kinase 1 , PCTK1; FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE; serine/threonine protein kinase; PCTAIRE-motif protein kinase 1; cell division protein kinase 16; serine/threonine-protein kinase PCTAIRE-1; PCTK1
• Gene ID: 5127
• mRNA Refseq: NM_001170460
• Protein Refseq: NP_001163931
• MIM: 311550
• UniProt ID: Q00536

Case Study

Case 1: Wei PL, et al. Int J Mol Sci. 2023

Colorectal cancer (CRC) is a major global health issue. Our study highlights the role of PCTK1, a kinase, in CRC. Patients with higher PCTK1 levels showed better survival rates. PCTK1 was found to curb cancer cell growth and stemness, while its absence increased tumor growth and drug resistance. These effects are linked to changes in apoptosis molecules and the BMPR1B-Smad signaling pathway. This pathway is crucial in managing cancer progression and treatment response, positioning PCTK1 as a promising target for improving CRC outcomes.

Fig1. The expression of BMPR1B in HCT 116 PCTK1-KO cells was determined through qPCR.

Fig2. PCTK1 expression of HCT 116 xenograft tumors was determined through Western blotting.

Case 2: Li X, et al. J Exp Clin Cancer Res. 2022

CDK16, a kinase linked to the Cyclin Y family, is found to be highly expressed in breast cancer, especially triple-negative breast cancer (TNBC). Its high levels are associated with worse patient outcomes. Research shows that CDK16 boosts TNBC cell growth and migration in lab settings and drives tumor growth and spread in living models. Knockdown or inhibition of CDK16 effectively slows down TNBC progression. CDK16’s role in tumor development involves phosphorylating PRC1, affecting mitosis. These findings suggest that targeting CDK16 could be a promising strategy for treating TNBC.

Fig1. Immunoblot analysis of CDK16 protein expression in clinical non-cancerous (N) and TNBC (T) samples.

Fig2. Immunoblot analysis of total Rb and p-Rb expression in MDA-MB-231 cells treated with CDK16-KD.

Application

CDK16, also known as PCTAIRE1 or PCTK1, is an uncommon cyclin-dependent kinase playing a role in cell cycle regulation, vesicle transport, neuron growth, and sperm development. Its recombinant form is pivotal in both research and industrial settings. In cancer studies, for instance, CDK16 shows abnormal activity across various cancers like lung, prostate, breast, melanoma, and liver. It's a key player in tumor growth and spread, acting as a potential marker for diagnosis and prognosis. Researchers leverage recombinant CDK16 to unravel its role in cancer by, for example, activating through Cyclin Y binding. In the field of neuroscience, CDK16 significantly influences neuron differentiation and axon/dendrite growth. Scientists utilize recombinant forms to dive into how it affects neural development and disorders. Apart from cancer and neuroscience, CDK16 is deeply involved in cell signaling by phosphorylation of substrates like NSF and CCNY, which impacts vesicle transport and signaling. Recombinant forms help decode these pathways. Moreover, CDK16 is central to drug discovery; it's pointed out that drugs like Dabrafenib inhibit its activity, paving the way for innovative cancer treatments. Its recombinant protein serves as a tool in vitro to probe its influence on the cell cycle, vesicle movement, and insulin release. On an industrial scale, recombinant CDK16 is efficiently produced in systems like E. coli or insect cells, ensuring high purity and low endotoxin, which is crucial for research purposes. As a cornerstone in diagnostics, CDK16's recombinant protein aids in developing specific antibodies for various detection methods, assessing its presence in different samples. Given its influence in cancer biology and beyond, CDK16 is instrumental for crafting drugs targeting it, such as inhibitors. Furthermore, its role in insulin secretion and glucose transport marks its importance in metabolic research. In reproductive biology, CDK16 is vital for sperm development, ensuring proper reproductive functions. Altogether, recombinant CDK16 is a valuable asset in cancer and neurobiology, signaling studies, drug development, and more, proving essential for cutting-edge research and crafting novel therapies.

Fig1. CDK16 participates in many biological functions through its interaction with many molecules and signaling pathways. (Xiao Wang, 2023)