Recombinant Human CDK12/CCNK Protein (Q696-S1082/M1-S300), GST/Flag-tagged

• Cat.No.: CDK12/CCNK-1661H
• Product Overview: Recombinant Human GST-TEV-CDK12(Q696-S1082)/pFastBac1-FLAG-TEV-Cyclink(1-300) protein with a GST/Flag was expressed in Insect cell

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&FLag
• ProteinLength: Q696-S1082/M1-S300
• Description: Cyclin-dependent kinase 12, Cdc2-related kinase, arginine/serine-rich, CrkRS, Cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, Cell division protein kinase 12, hCDK12; Cyclin-K Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors. Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).
• Form: Liquid
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH 8.0), 200 mM NaCl, 5% glycerol.
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: CDK12 cyclin-dependent kinase 12 [ Homo sapiens (human) ]
• Official Symbol: CDK12
• Synonyms: Cyclin-dependent kinase 12; Cdc2-related kinase; arginine/serine-rich; CrkRS; Cell division cycle 2-related protein kinase 7; CDC2-related protein kinase 7; Cell division protein kinase 12; hCDK12; Cyclin-K
• Gene ID: 51755
• mRNA Refseq: NM_015083
• Protein Refseq: NP_055898
• MIM: 615514
• UniProt ID: Q9NYV4