Recombinant Human CDC25A

• Cat.No.: CDC25A-27902TH
• Product Overview: Full-length recombinant Human Cdc25A with a N terminal proprietary tag. MWt 94 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Protein Length: 524 amino acids
• Description: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Weight: 94.000kDa inclusive of tags
• Biological activity: Specific activity 31 nmol phosphate released/min/mg.
• Form: Liquid
• Purity: Densitometry
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the MPI phosphatase family.Contains 1 rhodanese domain.

Gene Information

• Gene Name: CDC25A cell division cycle 25 homolog A (S. pombe) [ Homo sapiens ]
• Official Symbol: CDC25A
• Synonyms: CDC25A; cell division cycle 25 homolog A (S. pombe); cell division cycle 25 homolog A (S. cerevisiae) , cell division cycle 25A; M-phase inducer phosphatase 1
• Gene ID: 993
• mRNA Refseq: NM_001789
• Protein Refseq: NP_001780
• MIM: 116947
• Uniprot ID: P30304
• Chromosome Location: 3p21
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: hydrolase activity; protein binding; protein tyrosine phosphatase activity