Recombinant Human CDC25A

• Cat.No.: CDC25A-27898TH
• Product Overview: Recombinant full length Human Cdc25A; amino acids 1-524, 68kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-524 a.a.
• Description: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
• Biological activity: Activity:1 nmol of Cdc25A will hydrolyze 10 nmol of pNPP per minute at 37°C at pH 8.0.Assay buffer: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 0.1 mM EDTA.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 40mM Sodium chloride, 25mM HEPES, 2mM DTT, pH 7.2
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the MPI phosphatase family.Contains 1 rhodanese domain.
• Full Length: Full L.

Gene Information

• Gene Name: CDC25A cell division cycle 25 homolog A (S. pombe) [ Homo sapiens ]
• Official Symbol: CDC25A
• Synonyms: CDC25A; cell division cycle 25 homolog A (S. pombe); cell division cycle 25 homolog A (S. cerevisiae) , cell division cycle 25A; M-phase inducer phosphatase 1
• Gene ID: 993
• mRNA Refseq: NM_001789
• Protein Refseq: NP_001780
• MIM: 116947
• Uniprot ID: P30304
• Chromosome Location: 3p21
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: hydrolase activity; protein binding; protein tyrosine phosphatase activity