Recombinant Human CD8b molecule, His-tagged

• Cat.No.: CD8B-423H
• Product Overview: CD8B, 22-170aa, Human, His tag, E.coli

Specification

• Description: CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Recombinant human CD8B protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol
• Molecular Mass: 19.2kDa (172aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLQQTPAY IKVQTNKMVM LSCEAKISLS NMRIYWLRQR QAPSSDSHHE FLALWDSAKG TIHGEEVEQE KIAVFRDASR FILNLTSVKP EDSGIYFCMI VGSPELTFGK GTQLSVVDFL PTTAQPTKKS TLKKRVCRLP RPETQKGPLC SP
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Protein length: 22-170 a.a.

Gene Information

• Gene Name: CD8B CD8b molecule [ Homo sapiens ]
• Official Symbol: CD8B
• Synonyms: CD8B; CD8b molecule; CD8 antigen, beta polypeptide 1 (p37) , CD8B1; T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain; LY3; P37; LEU2; LYT3; CD8B1; MGC119115
• Gene ID: 926
• mRNA Refseq: NM_001178100
• Protein Refseq: NP_001171571
• MIM: 186730
• UniProt ID: P10966
• Chromosome Location: 2p12
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Disease, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem
• Function: MHC class I protein binding; coreceptor activity; protein binding