Recombinant Human CD80 Protein, Alexa Fluor 555 conjugated

• Cat.No.: CD80-590HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD80 extracellular domain (Met 1-Asn 242) (NP_005182.1), fused with five amino acids at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 214
• Form: Lyophilized
• Molecular Mass: The mature recombinant human B7-1 consists of 214 amino acids and predicts a molecular mass of 25.5 kDa. As a result of glycosylation, rhB7-1 migrates as approximately 45 kDa band in SDS-PAGE.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD80 CD80 molecule [ Homo sapiens ]
• Official Symbol: CD80
• Gene ID: 941
• mRNA Refseq: NM_005191
• Protein Refseq: NP_005182
• MIM: 112203
• UniProt ID: P33681