Recombinant Human CD80 Molecule, Fc-Tagged

• Cat.No.: CD80-54H
• Product Overview: Recombinant Human CD80 Molecule, Fc-Tagged expressed in human cells is a disulfide-linked homodimeric protein. The reduced monomer consists of 455 amino acids and predicts a molecular mass of 51.7 KDa. By SDS-PAGE, the apparent molecular mass of rh B7-1/Fc is approximately 80-90 KDa due to the glycosylation.

Specification

• Cat. No.: CD80-54H
• Description: B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulateT- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. A DNA sequence encoding the extracellular domain ( Met 1- Asn 242 ) of human B7-1 (NP_005182.1) precursor was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
• Predicted N terminal: Val 35
• Purity: > 95%, as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per 1μg cytokine as determined by the LAL method
• Formulation: Supplied as a 0.2μm filtered solution of PBS, pH7.4
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CD80
• Synonyms: CD28LG; CD28LG1; LAB7; B7; BB1; B-lymphocyte activation antigen B7; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; Activation B7-1 antigen;CD80; CD80 molecule
• Gene ID: 941
• mRNA Refseq: NM_005191
• Protein Refseq: NP_005182
• MIM: 112203
• UniProt ID: P33681
• Chromosome Location: 3q13.3-q21
• Pathway: Cell adhesion molecules (CAMs);Toll-like receptor signaling pathway; Type I diabetes mellitus
• Function: coreceptor activity; protein binding; transcription activator activity.