Recombinant Human CD70 protein, His-tagged, Biotinylated(Primary Amine Labeling)

• Cat.No.: CD70-5764H
• Product Overview: Biotinylated Recombinant Human CD70 protein(P32970-1)(Leu50-Pro193)(Primary Amine Labeling), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His
• Protein length: Leu50-Pro193
• Conjugation/Label: Biotin; Primary Amine
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD27, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer) , His Tag with the EC50 of 9.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD70 CD70 molecule [ Homo sapiens ]
• Official Symbol: CD70
• Synonyms: CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7
• Gene ID: 970
• mRNA Refseq: NM_001252
• Protein Refseq: NP_001243
• MIM: 602840
• UniProt ID: P32970