Recombinant Human CD4 protein, His-tagged

• Cat.No.: CD4-1559H
• Product Overview: Recombinant Human CD4 aa. (Lys26~Trp390) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Lys26~Trp390
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 9.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens (human) ]
• Official Symbol: CD4
• Synonyms: CD4mut; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
• Gene ID: 920
• mRNA Refseq: NM_000616.4
• Protein Refseq: NP_000607.1
• UniProt ID: P01730