Active Recombinant Human CD274 protein, His/Fc-tagged, Biotinylated

• Cat.No.: CD274-8230H
• Product Overview: Recombinant Human CD274(Phe 19 - Arg 238) fused with His/Fc tag at C-terminal was expressed in HEK293. Biotinylated.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Phe 19 - Arg 238
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human PD-1, Fc Tag at 2 μg/mL can bind Biotinylated Human PD-L1, His Tag & Fc Tag with a linear range of 0.01-1 μg/mL.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated Human PD-L1, His Tag & Fc tag by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Applications: ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7
• Chromosome Location: 9p24.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem
• Function: protein binding; protein tyrosine phosphatase activity; receptor activity