Recombinant Human CD274 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD274-592HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD274 extracellular domain N-terminal segment (Met 1-Thr 239) (NP_054862.1), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 232
• Form: Lyophilized
• Molecular Mass: The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the rh B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7