Recombinant Human CCR4-NOT transcription complex, subunit 8, His-tagged

• Cat.No.: CNOT8-207H
• Product Overview: CNOT8, 1-292aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-292 a.a.
• Description: CNOT8 belongs to the CAF1 family. It is a ubiquitous transcription factor required for a diverse set of processes and functions as part of the CCR-NOT complex. The CCR4-NOT complex functions as general transcription regulation complex. This complex contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8 and binds BTG2. Recombinant human CNOT8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 35.9 kDa (315aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPAALVE NSQVICEVWA SNLEEEMRKI REIVLSYSYI AMDTEFPGVV VRPIGEFRSS IDYQYQLLRC NVDLLKIIQL GLTFTNEKGE YPSGINTWQF NFKFNLTEDM YSQDSIDLLA NSGLQFQKHE EEGIDTLHFA ELLMTSGVVL CDNVKWLSFH SGYDFGYMVK LLTDSRLPEE EHEFFHILNL FFPSIYDVKY LMKSCKNLKG GLQEVADQLD LQRIGRQHQA GSDSLLTGMA FFRMKELFFE DSIDDAKYCG RLYGLGTGVA QKQNEDVDSA QEKMSILAII NNMQQ
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CNOT8 CCR4-NOT transcription complex, subunit 8 [ Homo sapiens ]
• Official Symbol: CNOT8
• Synonyms: CNOT8; CCR4-NOT transcription complex, subunit 8; POP2; CCR4-NOT transcription complex subunit 8; CAF1; CALIF; hCAF1; PGK promoter directed over production; CAF2; CALIFp; CAF1-like protein; CCR4-associated factor 8
• Gene ID: 9337
• mRNA Refseq: NM_004779
• Protein Refseq: NP_004770
• MIM: 603731
• UniProt ID: Q9UFF9
• Chromosome Location: 5q31-q33
• Pathway: Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Gene Expression, organism-specific biosystem; RNA degradation, organism-specific biosystem; RNA degradation, conserved biosystem
• Function: nucleic acid binding; sequence-specific DNA binding transcription factor activity