Recombinant Human CCN2 protein, hFc-tagged

• Cat.No.: CCN2-1689H
• Product Overview: Recombinant Human CCN2 protein(Q5M8T4)(Gln27-Ala349), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: N-hFc
• ProteinLength: Gln27-Ala349
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CTGF, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 61.77 kDa. Due to glycosylation, the protein migrates to 50-55 kDa and 62-72 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.