Recombinant Human CBR4, His-tagged

• Cat.No.: CBR4-2876H
• Product Overview: Recombinant human CBR4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CBR4 belongs to the short-chain dehydrogenase/reductase family. The formation of heteroteramer with HSD17B8 has NADH-dependent3-ketoacyl-acyl carrier protein reductase activity for o- and p-quinones. It plays a role in biosynthesis of fatty acids in mitochondria and has broad substrate specificity and reduces 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro).
• Sequence: MGSSHHHHHH SSGLVPRGSH MDKVCAVFGG SRGIGRAVAQ LMARKGYRLA VIARNLEGAK AAAGDLGGDH LAFSCDVAKE HDVQNTFEEM EKHLGRVNFL VNAAGINRDG LLVRTKTEDM VSQLHTNLLG SMLTCKAAMR TMIQQQGGSI VNVGSIVGLK GNSGQSVYSA SKGGLVGFSR ALAKEVARKK IRVNVVAPGF VHTDMTKDLK EEHLKKNIPL GRFGETIEVA HAVVFLLESP YITGHVLVVD GGLQLIL.
• Purity: > 95% by SDS – PAGE.
• MW: 27.5kDa (257aa) confirmed by MALDI-TOF.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 5mM DTT, 200mM NaCl.
• Concentration: 0.5mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CBR4 carbonyl reductase 4 [ Homo sapiens ]
• Synonyms: CBR4; carbonyl reductase 4; FLJ14431, SDR45C1; 3-oxoacyl-[acyl-carrier-protein] reductase; OTTHUMP00000219599; OTTHUMP00000219638; carbonic reductase 4; carbonyl reductase family member 4; quinone reductase CBR4; short chain dehydrogenase/reductase family 45C, member 1
• Gene ID: 84869
• mRNA Refseq: NM_032783
• Protein Refseq: NP_116172
• UniProt ID: Q8N4T
• Chromosome Location: 4q32.3
• Function: NADPH binding; NADPH dehydrogenase (quinone) activity; protein binding; quinone binding