Recombinant Human Caspase 8, Apoptosis-Related Cysteine Peptidase

• Cat.No.: CASP8-404H

Specification

• Cat. No.: CASP8-404H
• Description: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis. caspase-8 presents a promising target to restore defective apoptosis programs in cancers in order to overcome resistance.
• Source: E. coli.
• State Of Matter: Frozen.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Molecular Weight: On SDS polyacrylamide gels, the recombinant active caspase 8 migrates as two polypeptides of 18 and 10 kDa. Based on N-terminal sequencing, the 217-479 starting polypeptide is cleaved after the aspartate corresponding to aspartate 384 of human caspase 8.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Tag: Non

Gene Information

• Gene Name: CASP8 caspase 8, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Synonyms: caspase 8, apoptosis-related cysteine peptidase; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; FLJ17672; MGC78473; CASP8; caspase 8; OTTHUMP00000163720; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; FADD-homologous ICE/CED-3-like protease; caspase 8, apoptosis-related cysteine protease; CASP-8; EC 3.4.22.61; OTTHUMP00000163718; OTTHUMP00000163719; OTTHUMP00000163720; MORT1-associated CED-3 homolog; ICE-like apoptotic protease 5; FADD-like ICE; Apoptotic protease Mch-5; Apoptotic cysteine protease
• Gene ID: 841
• mRNA Refseq: NM_001080124
• Protein Refseq: NP_001073593
• MIM: 601763
• UniProt ID: Q14790
• Chromosome Location: 2q33-q34
• Pathway: Alzheimer"s disease; Apoptosis; Huntington"s disease; NOD-like receptor signaling pathway; Pathways in cancer; RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway; p53 signaling pathway
• Function: cysteine-type endopeptidase activity; identical protein binding; peptidase activity