Recombinant Human casein kinase 1, alpha 1, His-tagged

• Cat.No.: CSNK1A1-513H
• Product Overview: CSNK1A1, 1-337aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-337 a.a.
• Description: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. CSNK1A1 participates in Wnt signaling and phosphorylates CTNNB1 at "Ser-45". It may play a role in segregating chromosomes during mitosis. Recombinant human CSNK1a1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 41 kDa (357aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE SKLYKILQGG VGIPHIRWYG QEKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKLFLIDFGL AKKYRDNRTR QHIPYREDKN LTGTARYASI NAHLGIEQSR RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK MSTPVEVLCK GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM LKQKAAQQAA SSSGQGQQAQ TPTGKQTDKT KSNMKGF
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CSNK1A1 casein kinase 1, alpha 1 [ Homo sapiens ]
• Official Symbol: CSNK1A1
• Synonyms: CSNK1A1; casein kinase 1, alpha 1; casein kinase I isoform alpha; CK1; CK1a; CK1alpha; CKIa; CKIalpha; clock regulator kinase; CKI-alpha; down-regulated in lung cancer; HLCDGP1; PRO2975
• Gene ID: 1452
• mRNA Refseq: NM_001025105
• Protein Refseq: NP_001020276
• MIM: 600505
• UniProt ID: P48729
• Chromosome Location: 5q32
• Pathway: Beta-catenin phosphorylation cascade, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Degradation of beta-catenin by the destruction complex, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity