Recombinant Human CARHSP1 protein(Met1-Ser147), His-tagged

• Cat.No.: CARHSP1-454H
• Product Overview: Recombinant Human CARHSP1(Q9Y2V2 ) (Met1-Ser147) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Protein length: Met1-Ser147
• Form: Lyophilized from sterile 50mM Tirs, 10% Glycerol, 200mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CARHSP1 consists of 162 amino acids and predicts a molecular mass of 17.7 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CARHSP1 calcium regulated heat stable protein 1, 24kDa [ Homo sapiens ]
• Official Symbol: CARHSP1
• Synonyms: CARHSP1; calcium regulated heat stable protein 1, 24kDa; calcium regulated heat stable protein 1 (24kD); calcium-regulated heat stable protein 1; CRHSP 24; CSDC1; calcium-regulated heat-stable protein (24kD); calcium-regulated heat-stable protein of 24 kDa; CRHSP-24; MGC111446
• Gene ID: 23589
• mRNA Refseq: NM_001042476
• Protein Refseq: NP_001035941
• UniProt ID: Q9Y2V2

Case Study

Case 1: Pfeiffer JR, et al. Mol Cell Biol. 2011

Researchers explored TNF-α regulation, identifying CARHSP1 through an RNA capture assay. Knocking down CARHSP1 reduced TNF-α production and mRNA stability, while introducing it increased 3'UTR luciferase expression. Blocking Akt or calcineurin, but not PP2A, affected CARHSP1 function. CARHSP1 is found in processing bodies and exosomes and is critical for stabilizing TNF-α mRNA.

Fig1. Time course of CARHSP1 expression in resting and LPS-stimulated RAW264.7 cells.

Fig2. Expression of 100 ng of recombinant CARHSP1 in RAW264.7 cells.

Case 2: Li X, et al. Sci Rep. 2016

Researchers are studying miR-155's role in atherosclerosis, noting its increased presence in foam cells and patient samples. It targets CARHSP1, affecting TNF-α mRNA stability, and is elevated by NF-κB due to TNF-α. miR-155 helps curb inflammation via a feedback loop, offering protection in atherosclerosis through the miR-155-CARHSP1-TNF-α pathway.

Fig1. TNF-αmRNA half-life (t1/2) in cells treated with actinomycin D (ActD) post transfection with CARHSP1 siRNA or the control.

Fig2. Western blot analysis and Oil Red O staining were employed to examine the protein level of CARHSP1 and TNF-α.

Application

In the field of scientific research, CARHSP1 protein plays a vital role in gene expression and regulation. It influences glucose metabolism by inhibiting PPARγ-mediated transcription of gluconeogenesis genes and is known to bind mRNA, thus regulating its stability. This suggests its importance in mRNA stability and translational control. Additionally, CARHSP1's expression levels are tied to various diseases like cancer and diabetes, being significantly elevated in breast cancer and glioblastoma, which correlates with patient survival rates. In diabetes-related studies, it's explored as a biomarker for insulin resistance and complications. In proteomics, CARHSP1 has been used to understand protein interactions and cellular functions, such as its direct interaction with proteins like FARSA, unveiling its role in regulating mRNA stability within cells. In industrial production, CARHSP1 is extensively used as a recombinant protein in biopharmaceuticals and diagnostic tool development. Companies like Shanghai Baili Biotechnology offer plasmid products for its large-scale production, while others like Taosu Bio provide services for recombinant expression of CARHSP1, aiding in disease model construction, drug discovery, and functional research. Its applications stretch to industrial biotechnology, where it's crucial for enzyme activity studies, protein-protein interaction research, and protein structure analysis, all of which contribute to the development of new industrial enzymes and enhancements of existing processes. In clinical research, CARHSP1 is gaining attention as a potential biomarker and therapeutic target. It's identified as a stabilizing factor for TNF-α mRNA, playing a key role in inflammation and immune regulation, and is used in studies related to cancer patient survival and prognosis. Moreover, in radiation therapy, CARHSP1 is seen as a critical factor in the radioresistance of glioblastoma cells. Using gene-editing technologies like CRISPR/Cas9, researchers have pinpointed CARHSP1 as a gene influencing tumor radio-sensitivity. Overall, CARHSP1 offers vast potential across scientific research, industrial production, and clinical applications, making it a significant tool in life sciences research.

Fig1. Molecular structure of CRHSP-24 and its asymmetry. (Hai Hou, 2011)