Recombinant Human carbonic anhydrase XI, His-tagged

• Cat.No.: CA11-265H
• Product Overview: CA11, 24-328aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 24-328 a.a.
• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA11 is likely a secreted protein, however, radical changes at active site residues completely conserved in CA isozymes with catalytic activity, make it unlikely that it has carbonic anhydrase activity. It shares properties in common with two other acatalytic CA isoforms, CA VIII and CA X. CA11 is most abundantly expressed in brain, and may play a general role in the central nervous system. Recombinant human CA11 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Molecular Mass: 36.3kDa (326aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MHIGPAPDPE DWWSYKDNLQ GNFVPGPPFW GLVNAAWSLC AVGKRQSPVD VELKRVLYDP FLPPLRLSTG GEKLRGTLYN TGRHVSFLPA PRPVVNVSGG PLLYSHRLSE LRLLFGARDG AGSEHQINHQ GFSAEVQLIH FNQELYGNFS AASRGPNGLA ILSLFVNVAS TSNPFLSRLL NRDTITRISY KNDAYFLQDL SLELLFPESF GFITYQGSLS TPPCSETVTW ILIDRALNIT SLQMHSLRLL SQNPPSQIFQ SLSGNSRPLQ PLAHRALRGN RDPRHPERRC RGPNYRLHVD GVPHGR
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CA11 carbonic anhydrase XI [ Homo sapiens ]
• Official Symbol: CA11
• Synonyms: CA11; carbonic anhydrase XI; carbonic anhydrase-related protein 11; CA RP XI; carbonic anhydrase related protein 2; carbonic anhydrase related protein XI; CARP2; CARPX1; CA-XI; CARP-2; CARP XI; CA-RP II; carbonic anhydrase-related protein 2; carbonic anhydrase-related protein XI
• Gene ID: 770
• mRNA Refseq: NM_001217
• Protein Refseq: NP_001208
• MIM: 604644
• UniProt ID: O75493
• Chromosome Location: 19q13.3
• Function: NOT carbonate dehydratase activity