Recombinant Human CAPN14 HEK293T cell lysate

• Cat.No.: CAPN14-02HCL
• Product Overview: Human CAPN14 was derived in Human HEK293T cell line.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity (Dear et al., 2000 [PubMed 10964513]). CAPN14 belongs to the calpain large subunit family.
• Form: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Molecular Mass: 79.4 kDa
• Storage: Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: CAPN14 calpain 14 [ Homo sapiens ]
• Official Symbol: CAPN14
• Synonyms: CAPN14; calpain 14
• Gene ID: 440854
• mRNA Refseq: NM_001145122
• Protein Refseq: NP_001138594
• MIM: 610229
• UniProt ID: A8MX76