Recombinant Human CAPN11 protein, His & T7-tagged

• Cat.No.: CAPN11-1718H
• Product Overview: Recombinant Human CAPN11 aa. (Phe539~Gly739 (Accession # Q9UMQ6)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Phe539~Gly739
• Description: Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis.
• Form: Freeze-dried powder
• Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 5.3

Gene Information

• Gene Name: CAPN11 calpain 11 [ Homo sapiens (human) ]
• Official Symbol: CAPN11
• Synonyms: CAPN11; calpain 11; calpain11; calpain-11; CANP 11; calcium-activated neutral proteinase 11
• Gene ID: 11131
• mRNA Refseq: NM_007058.3
• Protein Refseq: NP_008989.2
• UniProt ID: Q9UMQ6