Recombinant Human CALY protein, His & T7-tagged

• Cat.No.: CALY-1100H
• Product Overview: Recombinant Human CALY aa. (Met1~Asn63 (Accession # Q9NYX4) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a type II single transmembrane protein. It is required for maximal stimulated calcium release after stimulation of purinergic or muscarinic but not beta-adrenergic receptors. The encoded protein interacts with D1 dopamine receptor and may interact with other DA receptor subtypes and/or GPCRs.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 10.4kDa.
• Protein length: Met1~Asn63
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: CALY calcyon neuron specific vesicular protein [ Homo sapiens (human) ]
• Official Symbol: CALY
• Synonyms: CALY; calcyon neuron specific vesicular protein; NSG3; DRD1IP; neuron-specific vesicular protein calcyon; D1 dopamine receptor-interacting protein; calcyon D1 dopamine receptor-interacting protein (CALCYON); calcyon protein; dopamine receptor D1 interacting protein
• Gene ID: 50632
• mRNA Refseq: NM_015722.3
• Protein Refseq: NP_056537.1
• UniProt ID: Q9NYX4