Recombinant Human CALD1 protein, His & T7-tagged

• Cat.No.: CALD1-6814H
• Product Overview: Recombinant Human CALD1 aa. (Gln28~Gln207 (Accession # Q05682)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Gln28~Gln207
• Description: This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.3kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: CALD1 caldesmon 1 [ Homo sapiens (human) ]
• Official Symbol: CALD1
• Synonyms: CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22; testis secretory sperm-binding protein Li 227n; caldesmon
• Gene ID: 800
• mRNA Refseq: NM_004342.6
• Protein Refseq: NP_004333.1
• UniProt ID: Q05682