Recombinant Human Calcium Activated Nucleotidase 1, His-tagged

Cat. No. :	CANT1-1338H
Description :	CANT1 (calcium-activated nucleotidase 1) belongs to the apyrase family. This protein is calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Also, it has very low activity towards ADP and even lower activity towards ATP. And it does not hydrolyze AMP and GMP. The specific function of CANT1 is as yet unknown, but its substrates are involved in several major signaling functions, including Ca2+ release, through activation of pyrimidinergic signaling.
Form :	Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol, 50 mM NaCl.
Molecular Weight :	40.5 kDa (364 aa), confirmed by MALDI-TOF
Purity :	> 90 % by SDS-PAGE
Concentration :	1 mg/ml (determined by Bradford assay)
Sequences of amino acids :	MGSSHHHHHH SSGLVPRGSH MGSHMRPAPG RPPTHNAHNW RLGQAPANWY NDTYPLSPPQ RTPAGIRYRI AVIADLDTES RAQEENTWFS YLKKGYLTLS DSGDKVAVEW DKDHGVLESH LAEKGRGMEL SDLIVFNGKL YSVDDRTGVV YQIEGSKAVP WVILSDGDGT VEKGFKAEWL AVKDERLYVG GLGKEWTTTT GDVVNENPEW VKVVGYKGSV DHENWVSNYN ALRAAAGIQP PGYLIHESAC WSDTLQRWFF LPRRASQERY SEKDDERKGA NLLLSASPDF GDIAVSHVGA VVPTHGFSSF KFIPNTDDQI IVALKSEEDS GRVASYIMAF TLDGRFLLPE TKIGSVKYEG IEFI
Storage :	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways :	Purine metabolism; Pyrimidine metabolism

Gene Name	CANT1 calcium activated nucleotidase 1 [ Homo sapiens ]
Official Symbol	CANT1
Synonyms	CANT1; calcium activated nucleotidase 1; DBQD; SCAN1; SHAPY; SCAN-1; soluble calcium-activated nucleotidase 1; apyrase homolog; putative MAPK-activating protein PM09; putative NF-kappa-B-activating protein 107; soluble Ca-activated nucleotidase, isozyme 1; soluble calcium-activated nucleotidase SCAN-1; Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase; micromelic dwarfism with vertebral and metaphyseal abnormalities and advanced carpotarsal ossification
Gene ID	124583
mRNA Refseq	NM_001159772
Protein Refseq	NP_001153244
MIM	613165
UniProt ID	Q8WVQ1
Chromosome Location	17q25.3
Function	calcium ion binding; hydrolase activity; nucleoside-diphosphatase activity; signal transducer activity

COA

Cat.No. :	CANT1-1338H
Product Overview :	Recombinant human CANT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.