Name: Recombinant Human C1orf65 293 Cell Lysate
SKU: C1orf65-8148HCL

Species :	Human
Source :	HEK293
Tag :	Non
Description :	Antigen standard for chromosome 1 open reading frame 65 (C1orf65) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Name	C1orf65 chromosome 1 open reading frame 65 [ Homo sapiens ]
Official Symbol	C1orf65
Synonyms	C1ORF65; chromosome 1 open reading frame 65; uncharacterized protein C1orf65; FLJ35728; RP11-76K24.1;
Gene ID	164127
mRNA Refseq	NM_152610
Protein Refseq	NP_689823
UniProt ID	Q8N715
Chromosome Location	1q42.11