Recombinant Human C12ORF5, His-tagged

• Cat.No.: C12ORF5-30300TH
• Product Overview: Recombinant full length Human TIGAR with an N terminal His tag; 294 amino acids with the tag, predicted mwt: 32.6 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 270 amino acids
• Description: This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
• Conjugation: HIS
• Molecular Weight: 32.600kDa inclusive of tags
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGSHMARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR
• Sequence Similarities: Belongs to the phosphoglycerate mutase family.

Gene Information

• Gene Name: C12orf5 chromosome 12 open reading frame 5 [ Homo sapiens ]
• Official Symbol: C12ORF5
• Synonyms: C12ORF5; chromosome 12 open reading frame 5; probable fructose-2,6-bisphosphatase TIGAR; TIGAR; TP53 induced glycolysis and apoptosis regulator
• Gene ID: 57103
• mRNA Refseq: NM_020375
• Protein Refseq: NP_065108
• MIM: 610775
• Uniprot ID: Q9NQ88
• Chromosome Location: 12p13.32
• Pathway: Direct p53 effectors, organism-specific biosystem; Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem
• Function: fructose-2,6-bisphosphate 2-phosphatase activity; hydrolase activity