Recombinant Human BTN3A1 protein, His-tagged

• Cat.No.: BTN3A1-47H
• Product Overview: Recombinant Human BTN3A1 protein(O00481-1)(Gln30-Gly254), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln30-Gly254
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human BTN3A1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: BTN3A1 butyrophilin, subfamily 3, member A1 [ Homo sapiens ]
• Official Symbol: BTN3A1
• Synonyms: BTN3A1; butyrophilin, subfamily 3, member A1; butyrophilin subfamily 3 member A1; BT3.1; BTF5; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1); MGC141880
• Gene ID: 11119
• mRNA Refseq: NM_001145008
• Protein Refseq: NP_001138480
• MIM: 613593
• UniProt ID: O00481