Recombinant Human BTK Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: BTK-398HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human BTK (NP_000052.1) (Met 1-Ser 659), fused with a C-terminal polyhistidine tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• ProteinLength: 670
• Form: Lyophilized
• Molecular Mass: The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 kDa. The apparent molecular mass of the protein is approximately 76 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 75 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Official Symbol: BTK
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187