Recombinant Human BRDT Protein (N21-E137), Tag Free

• Cat.No.: BRDT-1248H
• Product Overview: Recombinant Human BRDT(N21-E137) Protein was expressed in E. coli.

Specification

• Description: Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
• Source: E. coli
• Species: Human
• Tag: Non
• Form: Liquid
• Protein length: N21-E137
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: BRDT bromodomain, testis-specific [ Homo sapiens (human) ]
• Official Symbol: BRDT
• Synonyms: BRDT; bromodomain, testis-specific; bromodomain testis-specific protein; BRD6; cancer/testis antigen 9; CT9; RING3-like protein
• Gene ID: 676
• mRNA Refseq: NM_001242805
• Protein Refseq: NP_001229734
• MIM: 602144
• UniProt ID: Q58F21