Recombinant Human BRD3 protein, His&FLAG-tagged

• Cat.No.: BRD3-2838H
• Product Overview: Recombinant Human BRD3 protein (NM_007371.3)(24-144 aa) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His
• Protein Length: 24-144 aa
• Form: Supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol.
• Bio-activity: 3.3 μM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRD3 (24-144) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
• Molecular Mass: 20.5 kDa
• Purity: >90% pure by SDS-PAGE
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BRD3 bromodomain containing 3 [ Homo sapiens ]
• Official Symbol: BRD3
• Synonyms: BRD3; bromodomain containing 3; bromodomain-containing protein 3; KIAA0043; ORFX; RING3 like; RING3L; RING3-like protein; bromodomain-containing 3; FLJ23227; FLJ41328
• Gene ID: 8019
• mRNA Refseq: NM_007371
• Protein Refseq: NP_031397
• MIM: 601541
• UniProt ID: Q15059