Recombinant Human BPTF, GST-tagged

• Cat.No.: BPTF-156H
• Product Overview: Recombinant Human BPTF bromodomain (2796-2907 aa) fused to a GST tag at the N-terminus

Specification

• Description: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BPTF is the largest component of the NURF chromatin remodeling complex. It includes adjacent PHD and bromodomains which recognize trimethylation of H3K4 or acetylation of lysines in histone 4, respectively. BPTF is an essential regulator of gene expression in early mouse embryos, and its knock-out is embryonic lethal. Duplication of the BPTF gene has been suggested to increase proliferation of cultured cancer cells, and aberrant BPTF copy numbers were found in 42% of the “NCI-60” panel of 60 human cancer cell lines. This protein product contains the bromodomain of BPTF.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Liquid. 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5 mM β-mercaptoethanol and 5% glycerol.
• Molecular Mass: 40.1 kDa (2796-2907 aa + NT GST Tag)
• Purity: ≥80%
• Storage: Store at -80°C. Avoid repeated freeze and thaw cycles. Stable for 1 year.
• Protein length: 2796-2907 a.a.

Gene Information

• Gene Name: BPTF bromodomain PHD finger transcription factor [ Homo sapiens ]
• Official Symbol: BPTF
• Synonyms: BPTF; bromodomain PHD finger transcription factor; FALZ, fetal Alzheimer antigen; nucleosome-remodeling factor subunit BPTF; FAC1; NURF301; fetal Alzheimer antigen; fetal Alz-50 clone 1 protein; fetal Alz-50 reactive clone 1; nucleosome remodeling factor, large subunit; bromodomain and PHD domain transcription factor; bromodomain and PHD finger-containing transcription factor; FALZ
• Gene ID: 2186
• mRNA Refseq: NM_004459
• Protein Refseq: NP_004450
• MIM: 601819
• UniProt ID: Q12830
• Chromosome Location: 17q24
• Function: contributes_to DNA-dependent ATPase activity; metal ion binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding; transcription factor binding; zinc ion binding