Recombinant Human Betacellulin, His-tagged

• Cat.No.: BTC-869H
• Product Overview: Recombinant human BTC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 11.3 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 32-111aa
• Description: BTC (Betacellulin) is a member of the EGF family of growth factors and contains 1 EGF-like domain. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. The effects of BTC are probably mediated by the EGF receptor and other related receptors.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 200mM NaCl.
• Molecular Mass: 11.3 kDa (101aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MDGNSTRSPE TNGLLCGDPE ENCAATTTQS KRKGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYI GARCERVDLF Y
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BTC betacellulin [ Homo sapiens ]
• Official Symbol: BTC
• Synonyms: BTC; betacellulin; probetacellulin
• Gene ID: 685
• mRNA Refseq: NM_001729
• Protein Refseq: NP_001720
• MIM: 600345
• UniProt ID: P35070
• Chromosome Location: 4q13-q21
• Pathway: ErbB receptor signaling network, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem; ErbB4 signaling events, organism-specific biosystem; GRB2 events in ERBB2 signaling, organism-specific biosystem; Nuclear signaling by ERBB4, organism-specific biosystem
• Function: epidermal growth factor receptor binding; growth factor activity