Recombinant Human BEST2 protein, His & T7-tagged

• Cat.No.: BEST2-1714H
• Product Overview: Recombinant Human BEST2 aa. (Leu320~Ala509 (Accession # Q8NFU1)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 2 gene is mainly expressed in the retinal pigment epithelium and colon.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu320~Ala509
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 4.1

Gene Information

• Gene Name: BEST2 bestrophin 2 [ Homo sapiens (human) ]
• Official Symbol: BEST2
• Synonyms: BEST2; bestrophin 2; VMD2L1; bestrophin-2; VMD2-like gene 1; vitelliform macular dystrophy 2-like 1; vitelliform macular dystrophy 2-like protein 1
• Gene ID: 54831
• mRNA Refseq: NM_017682.2
• Protein Refseq: NP_060152.2
• UniProt ID: Q8NFU1