Recombinant Human basic helix-loop-helix family, member e40 Protein, His&StrepII tagged

• Cat.No.: BHLHE40-044H
• Product Overview: Recombinant human BHLHE40 protein (O14503) (Asp 193-Asp 412) with a Strep II tag at the N-terminus and a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&Strep II
• Protein Length: 193-412aa
• Description: Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity (PubMed:19786558). May be involved in the regulation of chondrocyte differentiation via the cAMP pathway. Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-BMAL1 complex. Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system.
• Tag: N-StrepII and C-His
• Molecular Mass: 26.9 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: BHLHE40 basic helix-loop-helix family, member e40 [ Homo sapiens (human) ]
• Official Symbol: BHLHE40
• Synonyms: BHLHE40; basic helix-loop-helix family, member e40; basic helix loop helix domain containing, class B, 2, BHLHB2, STRA13; class E basic helix-loop-helix protein 40; differentiated embryo chondrocyte expressed gene 1; bHLHe40; DEC1; differentially expressed in chondrocytes 1; class B basic helix-loop-helix protein 2; stimulated by retinoic acid gene 13 protein; enhancer-of-split and hairy-related protein 2; differentially expressed in chondrocytes protein 1; basic helix-loop-helix domain containing, class B, 2; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2; FLJ99214
• Gene ID: 8553
• mRNA Refseq: NM_003670
• Protein Refseq: NP_003661
• MIM: 604256
• UniProt ID: O14503