Recombinant Human BAD, GST-tagged

• Cat.No.: BAD-6973H
• Product Overview: Recombinant full-length human BAD was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full length
• Description: BAD is a member of the BCL-2 family of proteins that are known to be regulators of programmed cell death. BAD is a pro-apoptotic protein that forms a heterodimer complex with BCL-xL and BCL-2 which reverses the prosurvival activity of these proteins. The proapoptotic activity of BAD is regulated through its phosphorylation and this inhibits the pro-apoptosis function of BAD. Protein kinases such as AKT, RAF and RSK1 can phosphorylate BAD and RSK1-induced phosphorylation of BAD at ser112 suppresses BAD-mediated apoptosis in neurons. BAD inhibits G(1) to S phase transition in MCF7 breast cancer cells and overexpression of BAD inhibits cell growth as well as cyclin D1 expression.
• Form: Recombinant protein stored in 50mM Tris-150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25m DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~47 kDa
• Purity: >85%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2 μg/μl

Gene Information

• Gene Name: BAD BCL2-associated agonist of cell death [ Homo sapiens ]
• Official Symbol: BAD
• Synonyms: BAD; BCL2-associated agonist of cell death; bcl2 antagonist of cell death; BBC2; BCL2L8; bcl2-L-8; BCL2-binding protein; bcl-2-like protein 8; BCL2-binding component 6; bcl-2-binding component 6; BCL-X/BCL-2 binding protein; BCL2-antagonist of cell death protein; bcl-XL/Bcl-2-associated death promoter
• Gene ID: 572
• mRNA Refseq: NM_004322
• Protein Refseq: NP_004313
• MIM: 603167
• UniProt ID: Q92934
• Chromosome Location: 11q13.1
• Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Alpha-synuclein signaling, organism-specific biosystem
• Function: cysteine-type endopeptidase activator activity involved in apoptotic process; lipid binding; phospholipid binding; protein binding; protein heterodimerization activity; protein kinase binding; protein phosphatase binding