Recombinant Human B4GALT5, His-tagged

• Cat.No.: B4GALT5-42H
• Product Overview: Recombinant Human β-1,4-Galactosyltransferase 5/B4GALT5 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Pro36-Tyr388) of Human B4GALT5 fused with a polyhistidine tag at the C-terminus.

Specification

• Description: β-1,4-Galactosyltransferase 5 (B4GALT5) is a single-pass type II membrane protein that belongs to the glycosyltransferase 7 family. B4GALT5 includes a cytoplasmic domain (aa 1-14), a transmembrane segment (aa 15-35), and an extracellular region (aa36 - 388). B4GALT5 is ubiquitously expressed in many tissues and required for Manganese as cofactor. The B4GALT5 function is responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Supplied as a 0.2 μM filtered solution of 20mM HEPES, 150mM NaCl, 10% Glycerol, pH 7.5
• AA Sequence: PGIVNTYLFMMQAQGILIRDNVRTIGAQVYEQVLRSAYAKRNSSVNDSDYPLDLNHSETFLQTTT FLPEDFTYFANHTCPERLPSMKGPIDINMSEIGMDYIHELFSKDPTIKLGGHWKPSDCMPRWKVA ILIPFRNRHEHLPVLFRHLLPMLQRQRLQFAFYVVEQVGTQPFNRAMLFNVGFQEAMKDLDWDCL IFHDVDHIPESDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTVEQFRKINGFPNAFWG WGGEDDDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKERQGLDGLNNLN YFANITYDALYKNITVNLTPELAQVNEYVDHHHHHH
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Store at Please minimize freeze-thaw cycles.
• Protein length: 36-388 a.a.

Gene Information

• Gene Name: B4GALT5 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 [ Homo sapiens ]
• Official Symbol: B4GALT5
• Synonyms: B4GALT5; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5; beta-1,4-galactosyltransferase 5; beta4 GalT IV; beta4GalT V; beta4-GalT IV; beta-1,4-GalT II; beta-1,4-GalT IV; beta-1,4-GalTase 5; beta-1.4-galactosyltransferase V; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5; gt-V; B4Gal-T5; beta4Gal-T5; beta4GalT-V; BETA4-GALT-IV; MGC138470
• Gene ID: 9334
• mRNA Refseq: NM_004776
• Protein Refseq: NP_004767
• MIM: 604016
• UniProt ID: O43286
• Chromosome Location: 20q13.1-q13.2
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Mucin type O-Glycan biosynthesis, organism-specific biosystem; Mucin type O-Glycan biosynthesis, conserved biosystem; N-Glycan antennae elongation, organism-specific biosystem; N-glycan antennae elongation in the medial/trans-Golgi, organism-specific biosystem; O-linked glycosylation of mucins, organism-specific biosystem
• Function: galactosyltransferase activity; metal ion binding; transferase activity, transferring glycosyl groups